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News Physiol Sci 1: 5-8, 1986;
1548-9213/86 $5.00
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News in Physiological Sciences, Vol 1, 5-8, Copyright © 1986 by International Union of Physiological Sciences

ARTICLES

The Patch-Clamp Technique: Recording Ionic Currents Through Single Pores in the Cell Membrane

OH Petersen and CCH Petersen

When an intracellular electrode records an electrical event in the cell membrane, we know that there are changes in the membrane's permeability to various ions. These changes are mediated by membrane entities known as pores or ion channels. The advance of the patch-clamp technique, which permits the study of changes in individual ion channels, in the simplest case conforms to a simple open-close two-state model.




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