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J. Alvarez, M. Montero and J. García-Sancho are in the Instituto de Biología y Genética Molecular (IBGM), Departamento de Fisiología y Bioquímica, Facultad de Medicina, Universidad de Valladolid y Consejo Superior de Investigaciones Científicas, 47005-Valladolid, Spain.
The field of subcellular Ca2+ homeostasis is evolving rapidly. In parallel with improvements in spatial and temporal resolution of Ca2+ imaging techniques, new methods using the natural cell machinery to target Ca2+-sensitive proteins such as aequorin to precise intracellular locations promise superb specificity to measure [Ca2+] in defined subcellular environments.
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