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Measurement and Interpretation of Cytoplasmic [Ca²⁺] Signals From Calcium-Indicator Dyes

Stephen M. Baylor and Stephen Hollingworth

S. M. Baylor and S. Hollingworth are in the Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104-6085.
Ca²⁺-indicator dyes are widely used in biology yet difficult to characterize inside cells. Studies in skeletal muscle fibers provide important information about indicator behavior and about Ca²⁺ signaling within the cytoplasm.

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