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Physiology 21: 13-18, 2006; doi:10.1152/physiol.00045.2005
1548-9213/06 $8.00
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Physiology, Vol. 21, No. 1, 13-18, February 2006
© 2006 Int. Union Physiol. Sci./Am. Physiol. Soc.

EMERGING TECHNOLOGIES

Cryo-EM and Single Particles

Liguo Wang and Fred J. Sigworth

Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut

fred.sigworth{at}yale.edu

Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.






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