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Physiology 21: 6-12, 2006; doi:10.1152/physiol.00041.2005
1548-9213/06 $8.00
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Physiology, Vol. 21, No. 1, 6-12, February 2006
© 2006 Int. Union Physiol. Sci./Am. Physiol. Soc.

EMERGING TECHNOLOGIES

Patch Fluorometry: Shedding New Light on Ion Channels

Jie Zheng

Department of Physiology and Membrane Biology, University of California School of Medicine, Davis, California

jzheng{at}ucdavis.edu

Patch fluorometry has emerged as a new approach to the study of the structure-function relationship in membrane-embedded functional ion channels. Simultaneous fluorescent and electrical recordings are achieved from a small number of channels in a cell-free membrane patch, yielding high recording sensitivities. Further improvement of this approach should permit direct observation of the gating motion of a single-channel protein.






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