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Physiology 21: 162-170, 2006; doi:10.1152/physiol.00056.2005
1548-9213/06 $8.00
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Physiology, Vol. 21, No. 3, 162-170, June 2006
© 2006 Int. Union Physiol. Sci./Am. Physiol. Soc.

EMERGING TECHNOLOGIES

The Kindling Fluorescent Protein: A Transient Photoswitchable Marker

J. Nathan Henderson1,2 and S. James Remington2,3

1 Department of Chemistry,
2 Institute of Molecular Biology, and
3 Department of Physics, University of Oregon, Eugene, Oregon, jremington{at}uoxray.uoregon.edu

Passive fluorescent protein markers are indispensable for dynamic cellular imaging; however, they are unselective, introduce constant background fluorescence, and require continuous observation. Photoactivatable fluorescent proteins have now been developed whose fluorescence can be switched on and off by illumination, allowing selective and direct tracking of tagged objects without the need for continuous imaging. The "kindling fluorescent protein" is a photoactivatable marker with a novel twist: it turns itself off after a selectable period.






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