HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hofmann, A. F. Search for Related Content PubMed PubMed Citation Articles by Hofmann, A. F.

Bile Acids: The Good, the Bad, and the Ugly

Alan F. Hofmann

A. F. Hofmann is in the Division of Gastroenterology, Department of Medicine 0813, University of California, San Diego, CA 92093–0813.

	Abstract

 
Bile acids, amphipathic end products of cholesterol metabolism, are "good" in the infant because they enhance lipid absorption and thereby promote growth. Bile acids also induce bile flow and biliary lipid secretion. The enterohepatic circulation of bile acids is "bad" in the adult because it downregulates hepatocyte low-density lipoprotein receptor activity and thereby elevates plasma cholesterol levels. Defects in bile acid metabolism such as impaired biosynthesis or transport are "ugly" because they cause morbidity and death. New approaches for treating these defects are being developed.

	Introduction

Top Introduction The good The bad The ugly Conclusion References

 
In vertebrates, cholesterol balance is achieved by modulating both synthesis and excretion. Cholesterol excretion is mediated by bile acids, the water-soluble amphipathic molecules formed from cholesterol in the hepatocyte. In addition to their role in cholesterol homeostasis, bile acids also are functional detergents that induce bile flow and transport lipids as mixed micelles in the biliary tract and small intestine. Bile acid functions in health and dysfunctions in disease are the focus of this article.

	The good

Top Introduction The good The bad The ugly Conclusion References

 
Bile as a secretory and excretory fluid.
Bile, a fluid secreted by the liver into the intestine in vertebrates, is both a digestive and an excretory fluid. As a digestive fluid, bile contains bile acids, potent digestive surfactants that promote lipid absorption. As an excretory fluid, bile contains substances that cannot be eliminated efficiently in urine because they are insoluble or protein bound. These include not only bile acids (which are not only digestive surfactants but also end products of cholesterol metabolism), bilirubin (the end product of heme metabolism), cholesterol (derived from synthesis exceeding body needs), and heavy metals such as iron and copper (derived from absorption exceeding body needs). Biliary secretion also provides an excretory route for lipophilic steroids and drug metabolites. Bile also has a high concentration of phospholipids, which consist mostly of phosphatidylcholine (PC) and which form mixed micelles with bile acids. These mixed micelles contain amphipathic microdomains that can solubilize cholesterol. Mixed micelle formation also lowers the monomeric activity of bile acids and prevents their destroying the apical membrane of the biliary epithelial cells. IgA, an immunoglobulin, and mucus are secreted into bile, where their role is to prevent bacterial growth and adhesion. Finally, bile contains tocopherol, which may prevent oxidative damage to the biliary and small intestinal epithelium (5).

Bile acid metabolism.
Bile acids are formed in the pericentral hepatocytes from cholesterol by a multienzyme process. In the biosynthesis of C₂₄ bile acids (which are present in most vertebrates), the side chain of cholesterol undergoes oxidative cleavage resulting in the conversion of an isooctane moiety into an isopentanoic acid moiety. One or two hydroxyl groups are added to the nucleus. Although the pattern of hydroxylation varies between species, the hydroxylation is always on one face of the molecule, and the final product invariably has a hydrophobic face and a hydrophilic face, resulting in an amphipathic molecule. [Not all vertebrates form C₂₄ bile acids. In ancient mammals such as the elephant and manatee, as well as in cartilaginous fish, C₂₇ bile alcohols are formed. In reptiles, C₂₇ bile acids are formed (9). The topology of a typical C₂₄ bile acid is shown in Fig. 1.]

View larger version (37K): [in this window] [in a new window]   FIGURE 1 . Space-filling model of a conjugated bile acid molecule showing its biplanar, amphipathic nature because of a hydrophobic face and a hydrophilic face. The large size of the molecule and the charged end group make the bile acid anion impermeable to cell membranes and paracellular junctions. The amphipathic nature of the molecule is responsible for its forming mixed micelles with amphipathic but water-insoluble lipids such as phosphatidylcholine. From Ref. 15 with kind permission of Kluwer Academic Publishers.

Enhancement of dietary lipid absorption by bile acids.
Dietary triglyceride is hydrolyzed by pancreatic lipase to fatty acid and 2-monoglyceride molecules that are insoluble at physiological pH. The ability of bile acids to solubilize these lipolysis products efficiently has been known for nearly a century, and the discovery that such solubilization could be explained by mixed micelle formation was a superb application of concepts of colloid science to a physiological process. Recent work using neutron scattering indicates that the mixed micelles of bile (containing bile acids, PC, and cholesterol) and of small intestinal content (containing bile acids, fatty acids, and monoglyceride) have an identical molecular arrangement. In both types of micelles, the shape of the polymolecular aggregate is cylindrical. The polar lipids are arranged radially with their hydrophilic heads facing outward toward the aqueous phase. The bile acid molecules are arranged perpendicularly between their polar heads. The hydrophobic face of the bile acid molecule rests like a wedge between the heads of the alkyl chains of the PC (or fatty acid) molecules; the hydrophilic face of the bile acid molecule faces the aqueous environment (4). The transformation of lipid bilayers or vesicles to mixed micelles by bile acids is illustrated in Fig. 2.

View larger version (55K): [in this window] [in a new window]   FIGURE 2. Schematic depiction of the enterohepatic circulation of bile acids. Flux of bile acids is depicted by black lines and organs by stippled areas.

Bile acids will not solubilize dietary lipids in the form of mixed micelles unless bile acids are above a critical concentration, termed the critical micellization concentration. The relatively high concentration of bile acids in the small intestinal lumen during digestion is the result of several factors. First, conjugated bile acids are strong acids that are fully ionized at intestinal pH and are therefore impermeable to cell membranes, and the bile acid molecule is too large to pass across the paracellular junctions. Second, efficient conservation of bile acids by active (carrier mediated) absorption from the small intestine results in a pool of bile acids that cycles several times with each meal. (This movement of molecules from the biliary tract to the small intestine and back to the liver followed by resecretion into bile is termed the enterohepatic circulation.) The enterohepatic circulation of bile acids provides a large flux of surfactant molecules that greatly exceeds the rate at which bile acids are synthesized from cholesterol. For example, bile acid secretion with a meal averages 5 mmol/h. Synthesis of bile acids from cholesterol is ~0.02 mmol/h. The final factor contributing to the high luminal concentration of bile acids is the extremely high concentration of gallbladder bile (up to 300 mM), at least in those species that have gallbladders. The high concentration of gallbladder bile is the result of water removal by the gallbladder epithelium when bile is stored in the gallbladder.

The large recycling pool of bile acids thus serves a lipid transport function in both the biliary tract and the small intestine. In bile, the transport function of the "excretory" micelle (bile acids, PC, and cholesterol) helps in the excretion of cholesterol and other lipophilic molecules. In small intestinal content, the "absorptive" micelle helps in the absorption of triglyceride and fat-soluble vitamins. Both types of micelles can bind polyvalent cations and promote their transport.

Bile acid functions in the liver and biliary tract.
The large recycling pool of bile acids also has functions within the hepatocyte. Bile acids returning from the intestine are efficiently removed from portal venous blood by the hepatocyte. The first-pass extraction depends on the structure of the bile acid but for all bile acids exceeds 60%. Uptake is mediated by basolateral carrier proteins that are now being characterized at a molecular level (10). After uptake, bile acids do not remain in the hepatocyte but are rapidly pumped into the biliary canaliculus by an ATP-energized canalicular transporter that has recently been cloned (2). Transport across the canalicular membrane is extraordinarily concentrative, because the monomeric concentration of bile acids in the hepatocyte is thought to be 1–2 µM whereas that of canalicular bile is at least 1,000 µM. Bile acids are pumped into the canalicular space, which is semipermeable. As a result of the osmotic effects of the transiently elevated concentrations of bile acids, water and filtrable ions flow into the canalicular space, mostly via the paracellular junctions. In this manner, canalicular bile is generated. (The osmotic theory of bile formation was proposed about sixty years ago by Ivor Sperber and has been confirmed by multiple lines of experimental evidence; the mechanism of bile formation is identical to the pumping mechanism of the Alza pump.)

The canaliculi, which have a blind end at the pericentral region of the hepatic lobule, are surrounded by a spiral of actin microfilaments. These contract, driving canalicular bile toward the biliary ductules and initiating bile flow. After inducing bile flow into the canalicular lumen, bile acids also induce biliary lipid secreting by adsorbing to PC molecules that have been transported across the canalicular membrane by a PC-"flippase" (11). The PC molecules form vesicular bubbles that bud from the luminal face of the canalicular membrane, the energy for vesicle formation perhaps being provided by the canalicular contractions. Bile acids adsorb to these vesicles, detaching them from the luminal face of the canalicular membrane (11). As bile flows down the biliary tract, the vesicles are converted to mixed micelles by the continued adsorption of bile acid molecules. How cholesterol is transported into canalicular bile remains poorly understood. The conversion of vesicles to mixed micelles by bile acids is shown in Fig. 3.

View larger version (41K): [in this window] [in a new window]   FIGURE 3. Solubilization of vesicles or bilayers to form mixed micelles by bile acids (BA). Although mixed micelle is shown as a cylinder, at higher lipid-to-bile acid ratios, the micelle may elongate and become worm shaped. In bile, mixed micelle is composed mainly of conjugated bile acids, phosphatidylcholine, and cholesterol and is formed by bile acids adsorbing to the bilayer vesicles and transforming them into mixed micelles. In small intestinal content, mixed micelle is composed mainly of bile acids, monoglycerides, and fatty acids and is formed by bile acids adsorbing to lamellae of fatty acids and monoglycerides generated by pancreatic lipase on the surface of the triglyceride droplet. Lipid-soluble dietary constituents such as fat-soluble vitamins and cholesterol will also partition into mixed micelle that is present in small intestinal content.

Bile acid transport.
The enterohepatic circulation thus involves transcellular transport of bile acid molecules, mediated by membrane transporters, and interorgan flow, mediated by intestinal motility and blood flow. Vectorial membrane transport occurs in the hepatocyte and the ileal enterocyte, and interorgan flow occurs in the portal venous and systemic circulation, the biliary tract, and the small intestine.

The chemical pumps present in the hepatocyte have already been described. Evidence for the presence of an active transport system in the ileal enterocyte was provided by Lack and Weiner, who showed, using everted intestinal sacs, that conjugated bile acids move uphill in a Na⁺-dependent manner. Recently, work by Dawson and colleagues (1) has led to the cloning of the Na⁺-bile acid cotransporter present in the apical membrane of the ileal enterocyte. The ileal bile acid transporter shares homology with the hepatocyte Na⁺-dependent bile acid transporter located in the sinusoidal membrane but is a different protein. Exit of bile acids from the ileal enterocyte across the basolateral membrane and into portal venous blood involves a second carrier protein with anion-exchange products that remains poorly characterized.

The enterohepatic circulation of bile acids is under homeostatic control at the level of the both the hepatocyte, with respect to bile acid biosynthesis, and the ileal enterocyte, with respect to bile acid transport. At the hepatocyte level, a decreased return of bile acids to the hepatocyte is followed by increased bile acid biosynthesis; the signal appears to be the intracellular concentration of bile acids. Normally, bile acid synthesis is downregulated. With interruption of the enterohepatic circulation, bile acid biosynthesis increases up to 15-fold. Because bile acids are derived from cholesterol, increased bile acid biosynthesis must be accompanied by an equivalent amount of cholesterol biosynthesis.

Homeostasis at the ileal enterocyte level is not well understood. There is convincing evidence for negative feedback (decreased transport with increased load) in the hamster and guinea pig; negative feedback of ileal transport is also present in humans, because bile acid secretion does not increase when bile acids are fed.

	The bad

Top Introduction The good The bad The ugly Conclusion References

 
Inappropriate retention of bile acids and cholesterol metabolism.
The physiological utility of the enterohepatic circulation of bile acids has fascinated physiologists for centuries. Borelli, the legendary animal physiologist, predicted the existence of the enterohepatic circulation in the 17th century. Experimental proof of the enterohepatic circulation awaited studies in the biliary fistula dog in 1870 by Moritz Schiff in Geneva. (Schiff had been forced to abandon his laboratory in Florence, Italy and flee to Switzerland after being put on trial by antivivisectionists; at his trial, he made an eloquent defense of the need and moral justification for animal experimentation.)

The view that conjugated bile acids were functional detergent molecules that solubilized lipids, thereby promoting their absorption, was well accepted by physiologists a half-century ago even if mixed micelle formation had not yet been described. The enterohepatic circulation was considered an extraordinary physiological adaptation that made a large flux of detergent molecules available for digestion with minimal biosynthesis requirements. However, this view that the efficient enterohepatic circulation was "good" for the organism has been modified in the past decade, largely as a result of advances in understanding cholesterol and lipoprotein metabolism. It now seems increasingly likely that efficient intestinal conservation of bile acids also has a "bad" aspect. Brown and Goldstein, in their seminal work on the low-density lipoprotein (LDL) receptor, pointed out that impaired function of this receptor caused hypercholesterolemia. Upregulation of this receptor could be achieved by increasing the demands of the hepatocyte for cholesterol, because the hepatocyte appears to defend cholesterol homeostasis at all costs. They noted that bile acid sequestrants induced bile acid and cholesterol biosynthesis, which in turn upregulated LDL-receptor activity. Thus, in the adult, efficient intestinal conservation of bile acids may be considered bad in that it downregulates cholesterol biosynthesis and LDL-receptor activity, which in turn leads to higher plasma LDL cholesterol levels.

Even before the elegant depiction of Brown and Goldstein, pharmaceutical companies had identified the active transport of bile acids by the ileum as a physiological process that should be targeted for treating hypercholesterolemia. The first agent, the bile acid sequestrant cholestyramine, caused a threefold increase in cholesterol and bile acid biosynthesis and lowered plasma LDL cholesterol levels. Although cholestyramine has been shown to be safe and effective for the prevention of coronary events in patients with familial hypercholesterolemia, it is not widely used because of undesirable side effects such as constipation. More potent sequestrants with fewer side effects are being developed. A second approach is the synthesis of inhibitors of the Na⁺-bile acid cotransporter present on the apical membrane of the ileal enterocytes. Such agents have been shown to inhibit bile acid absorption, to lower plasma cholesterol, and to decrease atherosclerosis in the cholesterol-fed Watanabe rabbit that is known to have defective LDL receptors (13).

	The ugly

Top Introduction The good The bad The ugly Conclusion References

 
Bile acids as cytotoxic agents.
The amphipathic properties of bile acids that cause them to be such powerful solubilizers of membrane lipids are also responsible for bile acids being cytotoxic when present at abnormally high concentrations either intracellularly or extracellularly. The cytotoxic effects of bile acids may cause distressing symptoms or even death. This is the "ugly" side of bile acids.

Cytotoxicity caused by increased intracellular concentrations of bile acids.
In the healthy hepatocyte, uptake of bile acids across the basolateral membrane and export via the canalicular export pump are tightly coupled. This efficient coupling, together with the presence of binding proteins in the cytosol, keeps the monomeric concentrations of bile acids within the hepatocyte at extremely low concentrations, <3 µM. When canalicular export is defective either because of an inborn or acquired defect in canalicular transport or when there is a physical obstruction to bile flow, bile acids accumulate within the hepatocyte. When their concentration exceeds the binding capacity of the binding proteins, bile acids induce apoptosis and necrosis, probably by damage to mitochondria (14). In patients with cholestatic liver disease, the extent of hepatocyte damage caused by intracellular accumulation of bile acids can be decreased by ingesting a nontoxic bile acid (ursodiol), which accumulates in the circulating bile acid pool and decreases the cytotoxicity of the bile acid mixture circulating through the hepatocyte (12).

Certain inborn errors of bile acid biosynthesis lead to increased synthesis of bile acid precursors. These accumulate within the hepatocyte because they are not substrates for the canalicular export pump and cause hepatocyte death. Infants with these extremely rare conditions develop progressive jaundice after birth. The diagnosis is usually made by mass spectrometric identification of the intermediates in urine or plasma. Treatment with natural bile acids suppresses the synthesis of the toxic intermediates, induces normal bile flow, restores the concentration of bile acids in the small intestine, and is life saving.

In principle, bile acids should accumulate in the ileal enterocyte when basolateral transport is impaired, and such accumulation should cause enterocyte death. However, this situation has not as yet been induced experimentally or identified clinically.

Cytotoxicity caused by increased extracellular concentrations of bile acids.
In health, efficient ileal conservation, together with rapid bacterial modification of bile acids entering the colon, results in the aqueous concentration of bile acids in colonic contents being quite low, <1 mM. When ileal transport of bile acids is defective, either because of a congenital absence of the ileal bile acid transporter or because of ileal resection or disease, a compensatory increase in hepatic biosynthesis occurs, and a greatly increased amount of bile acids passes into the colon. The elevated intraluminal concentration induces secretion of electrolytes and water, manifested clinically as diarrhea. Administration of bile acid sequestrants lowers the elevated intraluminal concentration of bile acids and provides symptomatic benefit (6).

Bile acid deficiency states.
A bile acid deficiency in the intestine occurs when the enterohepatic circulation of bile acids is obstructed or when it is interrupted by impaired intestinal conservation. If bile acid malabsorption is sufficiently severe, the compensatory increase in bile acid biosynthesis is insufficient to restore bile acid secretion into the intestine. (In humans, the liver can increase its synthesis of bile acids only ~15-fold. The maximal rate of bile acid biosynthesis is ~6 g/day, which is less than one-half of the normal daily secretion rate of bile acids.) Decreased bile acid secretion leads to defective micellar solubilization of dietary lipid, and this contributes to lipid malabsorption in these patients. If conjugated bile acids are fed orally, micellar solubilization is restored and improved lipid absorption results. The clinical utility of such conjugated bile acid replacement therapy is being tested (3).

	Conclusion

Top Introduction The good The bad The ugly Conclusion References

 
Elucidation of the metabolism, enterohepatic circulation, and functions of bile acids as well as disturbances of these processes in disease has involved a multidisciplinary effort that has ranged from physical biochemistry to clinical trials. In my opinion, these efforts have been in the best traditions of integrative physiology.

	Acknowledgments

 
Helpful suggestions were made by Dr. Carolina Cerrè and Dr. Lee R. Hagey. Some of the material in this article was presented in the Horace W. Davenport Lecture in April, 1996. The author's work was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grant DK-21506 as well as a grant-in-aid from the Falk Foundation e.V., Freiburg, Germany.

	References

Top Introduction The good The bad The ugly Conclusion References

 

1. Craddock, A. L., M. W. Love, R. W. Daniel, L. C. Kirby, H. C. Walters, M. H. Wong, and P. A. Dawson. Expression and transport properties of the human ileal and renal sodium-dependent bile acid transporter. Am. J. Physiol. 274 (Gastrointest. Liver Physiol. 37): G157–G169, 1998.[Abstract/Free Full Text]
2. Gerloff, T., B. Stieger, B. Hagenbuch, J. Madon, L. Landmann, J. Roth, A. F. Hofmann, and P. J. Meier. The sister of P-glycoprotein represents the canalicular bile salt. J. Biol. Chem. 273: 10046–10050, 1998.[Abstract/Free Full Text]
3. Gruy-Kapral, C., K. H. Little, J. S. Fordtran, L. R. Hagey, and A. F. Hofmann. Cholylsarcosine improves fat absorption in short bowel syndrome. Gastroenterology 114: A1531, 1998.
4. Hjelm, R. P., C. D. Schteingart, A. F. Hofmann, and D. S. Sivia. Form and structure of self-assembling particles in monoolein-bile salt mixtures. J. Phys. Chem. 99: 16395–16400, 1995.
5. Hofmann, A. F. Overview of bile secretion. In Handbook of Physiology. The Gastrointestinal System. Salivary, Gastric, Pancreatic, and Hepatobiliary Secretion. Bethesda, MD: Am. Physiol. Soc., 1989, sect. 6, vol. III, chapt. 28, p. 549–566.
6. Hofmann, A. F. Bile secretion and the enterohepatic circulation of bile acids. In: Gastrointestinal Disease, edited by M. Feldman, B. F. Scharschmidt, and M. H. Sleisenger. Philadelphia, PA: Saunders, 1989, p. 937–948.
7. Hofmann, A. F., and L. R. Hagey. Bile acids and biliary disease: peaceful coexistence versus deadly warfare. In: Gut and Liver, edited by H. E. Blum, C. Bode, J. C. Bode, and R. B. Sartor. Lancaster, UK: Kluwer Academic, 1998, p. 85–103.
8. Hofmann, A. F., and K. J. Mysels. Bile salts as biological surfactants. Colloids Surfaces 30: 145–173, 1988.
9. Hofmann, A. F., C. D. Schteingart, and L. R. Hagey. Species differences in bile acid metabolism. In: Bile Acids in Liver Diseases, edited by G. Paumgartner and U. Beuers. Boston, MA: Kluwer Academic, 1995. p. 3–30.
10. Müller, M., and P. L. M. Jansen. Molecular aspects of hepatobiliary transport. Am. J. Physiol. 272 (Gastrointest. Liver. Physiol. 35): G1285–G1303, 1997.[Abstract/Free Full Text]
11. Oude Elferink, R. P. and A. K. Groen. The role of MDR2 p-glycoprotein in biliary lipid secretion—cross-talk between cancer research and biliary physiology. Hepatology 23: 617–628, 1995.
12. Poupon, R. E., K. D. Lindor, K. Cauch-Dudek, E. R. Dickson, R. Poupon, and E. J. Heathcote. Combined analysis of randomized controlled trials of ursodeoxycholic acid in primary biliary cirrhosis. Gastroenterology 113: 884–890, 1997.[Medline]
13. Princen, H. M. G., S. M. Post, and J. Twisk. Regulation of bile acid biosynthesis. Curr. Pharmaceut. Des. 3: 59–84, 1997.
14. Roberts, L. R., H. Kurosawa, S. F. Bronk, P. J. Fesmier, L. B. Agellon, W. Y. Leung, F. Mao, and G. J. Gores. Cathepsin B contributes to bile salt-induced apoptosis of rat hepatocytes. Gastroenterology 113: 1714–1726, 1997.[Medline]
15. Hofmann, A. F. Overview: enterohepatic circulation of bile acids—a topic in molecular physiology. In Bile Acids in Health and Disease, edited by T. Northfield, R. Jazrawi, and P. Zentler-Munro. Lancaster, UK: Kluwer Academic, 1988. p. 1–18.

This article has been cited by other articles:

				C. Zhao and K. Dahlman-Wright Liver X receptor in cholesterol metabolism J. Endocrinol., March 1, 2010; 204(3): 233 - 240. [Abstract] [Full Text] [PDF]

				M. Huertas, L. Hagey, A. F. Hofmann, J. Cerda, A. V. M. Canario, and P. C. Hubbard Olfactory sensitivity to bile fluid and bile salts in the European eel (Anguilla anguilla), goldfish (Carassius auratus) and Mozambique tilapia (Oreochromis mossambicus) suggests a `broad range' sensitivity not confined to those produced by conspecifics alone J. Exp. Biol., January 15, 2010; 213(2): 308 - 317. [Abstract] [Full Text] [PDF]

				J. Le lay and K. H. Kaestner The Fox Genes in the Liver: From Organogenesis to Functional Integration Physiol Rev, January 1, 2010; 90(1): 1 - 22. [Abstract] [Full Text] [PDF]

				D. Watson, R. D. Sleator, P. G. Casey, C. Hill, and C. G. M. Gahan Specific Osmolyte Transporters Mediate Bile Tolerance in Listeria monocytogenes Infect. Immun., November 1, 2009; 77(11): 4895 - 4904. [Abstract] [Full Text] [PDF]

				A. K. Deo and S. M. Bandiera 3-Ketocholanoic Acid Is the Major in Vitro Human Hepatic Microsomal Metabolite of Lithocholic Acid Drug Metab. Dispos., September 1, 2009; 37(9): 1938 - 1947. [Abstract] [Full Text] [PDF]

				I. L. Csanaky, L. M. Aleksunes, Y. Tanaka, and C. D. Klaassen Role of hepatic transporters in prevention of bile acid toxicity after partial hepatectomy in mice Am J Physiol Gastrointest Liver Physiol, September 1, 2009; 297(3): G419 - G433. [Abstract] [Full Text] [PDF]

				Y. Alnouti Bile Acid Sulfation: A Pathway of Bile Acid Elimination and Detoxification Toxicol. Sci., April 1, 2009; 108(2): 225 - 246. [Abstract] [Full Text] [PDF]

				R. W. Crawford, D. L. Gibson, W. W. Kay, and J. S. Gunn Identification of a Bile-Induced Exopolysaccharide Required for Salmonella Biofilm Formation on Gallstone Surfaces Infect. Immun., November 1, 2008; 76(11): 5341 - 5349. [Abstract] [Full Text] [PDF]

				D. C. Hissa, I. M. Vasconcelos, A. F. U. Carvalho, V. L. R. Nogueira, P. Cascon, A. S. L. Antunes, G. R. de Macedo, and V. M. M. Melo Novel surfactant proteins are involved in the structure and stability of foam nests from the frog Leptodactylus vastus J. Exp. Biol., August 15, 2008; 211(16): 2707 - 2711. [Abstract] [Full Text] [PDF]

				A. Birkenmaier, J. Holert, H. Erdbrink, H. M. Moeller, A. Friemel, R. Schoenenberger, M. J.-F. Suter, J. Klebensberger, and B. Philipp Biochemical and Genetic Investigation of Initial Reactions in Aerobic Degradation of the Bile Acid Cholate in Pseudomonas sp. Strain Chol1 J. Bacteriol., October 15, 2007; 189(20): 7165 - 7173. [Abstract] [Full Text] [PDF]

				R. S. Kumar, J. A. Brannigan, A. A. Prabhune, A. V. Pundle, G. G. Dodson, E. J. Dodson, and C. G. Suresh Structural and Functional Analysis of a Conjugated Bile Salt Hydrolase from Bifidobacterium longum Reveals an Evolutionary Relationship with Penicillin V Acylase J. Biol. Chem., October 27, 2006; 281(43): 32516 - 32525. [Abstract] [Full Text] [PDF]

				S W C van Mil, R H J Houwen, and L W J Klomp Genetics of familial intrahepatic cholestasis syndromes J. Med. Genet., June 1, 2005; 42(6): 449 - 463. [Abstract] [Full Text] [PDF]

				W. A. Alrefai, Z. Sarwar, S. Tyagi, S. Saksena, P. K. Dudeja, and R. K. Gill Cholesterol modulates human intestinal sodium-dependent bile acid transporter Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G978 - G985. [Abstract] [Full Text] [PDF]

				M. Begley, R. D. Sleator, C. G. M. Gahan, and C. Hill Contribution of Three Bile-Associated Loci, bsh, pva, and btlB, to Gastrointestinal Persistence and Bile Tolerance of Listeria monocytogenes Infect. Immun., February 1, 2005; 73(2): 894 - 904. [Abstract] [Full Text] [PDF]

				T. Kok, C. V. Hulzebos, H. Wolters, R. Havinga, L. B. Agellon, F. Stellaard, B. Shan, M. Schwarz, and F. Kuipers Enterohepatic Circulation of Bile Salts in Farnesoid X Receptor-deficient Mice: EFFICIENT INTESTINAL BILE SALT ABSORPTION IN THE ABSENCE OF ILEAL BILE ACID-BINDING PROTEIN J. Biol. Chem., October 24, 2003; 278(43): 41930 - 41937. [Abstract] [Full Text] [PDF]

				M. Trauner and J. L. Boyer Bile Salt Transporters: Molecular Characterization, Function, and Regulation Physiol Rev, April 1, 2003; 83(2): 633 - 671. [Abstract] [Full Text] [PDF]

				C. V. Hulzebos, L. Renfurm, R. H. Bandsma, H. J. Verkade, T. Boer, R. Boverhof, H. Tanaka, I. Mierau, P. J. J. Sauer, F. Kuipers, et al. Measurement of parameters of cholic acid kinetics in plasma using a microscale stable isotope dilution technique: application to rodents and humans J. Lipid Res., November 1, 2001; 42(11): 1923 - 1929. [Abstract] [Full Text] [PDF]

				A. Zsembery, T. Thalhammer, and J. Graf Bile Formation: a Concerted Action of Membrane Transporters in Hepatocytes and Cholangiocytes Physiology, February 1, 2000; 15(1): 6 - 11. [Abstract] [Full Text] [PDF]

				A. F. Hofmann The Continuing Importance of Bile Acids in Liver and Intestinal Disease Arch Intern Med, December 13, 1999; 159(22): 2647 - 2658. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hofmann, A. F. Search for Related Content PubMed PubMed Citation Articles by Hofmann, A. F.