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Ubiquitin-Dependent Proteolysis in Neurons

Lars Klimaschewski

Institute of Anatomy and Histology, Deptartment of Neuroanatomy, University of Innsbruck, A-6010 Innsbruck, Austria

	Abstract

 
Various studies identified the ubiquitin-proteasome system as the prime suspect in causing neurodegenerative diseases. The present review summarizes our current knowledge about the expression, regulation, and functions of this major protein degradation pathway in the brain, with particular reference to the pathogenesis of associated neurological diseases.

	Introduction

Top Introduction Relevance of ubiquitination for... Regulation and function of... Involvement of the UPS... Conclusions References

 
Ubiquitination of proteins and their degradation within the proteasome emerged as the major proteolytic mechanism used by mammalian cells to regulate cytosolic and nuclear protein levels. The ubiquitin-proteasome pathway eliminates a variety of normal and abnormal, often short-lived proteins, indicating the central role of the ubiquitin-proteasome system (UPS) in most cellular functions. Posttranslational protein modification by ubiquitin (ubiquitination or ubiquitylation) may be compared with another mechanism of protein modification: phosphorylation. Both mechanisms regulate each other; enzymes involved in the ubiquitination process or proteins destined to be degraded need in part to be phosphorylated. Alternatively, degradation of phosphatases through the UPS indirectly determines protein phosphorylation of various targets.

Several recent observations reveal the functional versatility of the UPS. For example, proteasomal degradation of precursors is required to yield biologically active transcription factors like NF-B. Second, ubiquitination not only allows proteins to be directed to the proteasome, but ubiquitin is also used to label membrane proteins or luminal components of the endoplasmic reticulum for degradation in lysosomes. On the other hand, the proteasome is involved in degradation of nonubiquitinated proteins in an ATP-independent fashion. Unrelated to proteolysis, monoubiquitination has been found to be involved in endosomal transport and transcriptional regulation. Hence the UPS plays an important role in a variety of basic cellular functions beyond those that aim at targeting and destruction of misfolded, mutated, or otherwise damaged proteins.

Ubiquitination is accomplished by a series of enzymatic steps that are required for covalent attachment of multiple ubiquitin molecules to the protein designated for degradation (Fig. 1). This process includes the ATP-dependent activation of ubiquitin by the ubiquitin-activating enzyme (E1), transfer of ubiquitin to a member of the ubiquitin-conjugating enzyme (E2) family, and isopeptide linkage of the 76-amino acid residue ubiquitin to a lysin residue of the target protein, which is then catalyzed by an E3 ubiquitin ligase (19). Differences among E2s, which are characterized by a conserved core domain, apparently determine the specificity of their interactions with E3s and their subcellular location. E3s confer specificity in the ubiquitination process. They express particular domains called HECT domains or RING fingers. HECT domains represent the ~350-amino-acid-residue carboxy-terminal sequence exhibiting a conserved cysteine for intermediate binding of ubiquitin, whereas RING fingers consist of eight metal-binding residues coordinating two zinc ions and serve as a docking site for E2s.

View larger version (12K): [in this window] [in a new window]   FIGURE 1. Simplified model of protein ubiquitination and degradation by the ubiquitin-proteasome system and its possible role in neuronal degeneration.

Deubiquitination emerged as an important and tightly regulated process similar to the removal of phosphate groups by phosphatases. Thiol proteases of the ubiquitin carboxy-terminal hydrolase and ubiquitin-specific processing enzyme family are required for removal of ubiquitin from carboxy-terminal fusion proteins or from multiubiquitin chains, respectively. They are likely to play a crucial role in separation of ubiquitin from the target protein before it is introduced into the proteasome cylinder, thereby facilitating proteasomal function and preventing the accumulation of ubiquitin chains. Recent evidence points to an important function of ubiquitin carboxy-terminal hydrolase as an immediate-early gene that is required for long-term facilitation in Aplysia neurons (7). This enzyme presumably leads to increased degradation of substrates that normally inhibit long-term memory formation.

	Relevance of ubiquitination for neuronal development

Top Introduction Relevance of ubiquitination for... Regulation and function of... Involvement of the UPS... Conclusions References

 
The distribution of ubiquitin as well as ubiquitin-conjugating, -ligating, and -hydrolyzing enzymes in the brain had not been elucidated until recently. For many years, however, neuroscientists used antibodies against the neuronal marker protein PGP9.5 to label central and peripheral neuron populations as well as the diffuse neuroendocrine system. The antigen was identified as a highly conserved ubiquitin carboxy-terminal hydrolase and is estimated to comprise 1–2% of the total soluble brain protein.

Ubiquitin levels are high during neuronal development, with strong staining in the dendritic compartment of early postnatal pyramidal neurons in the cortex and hippocampus as well as in Purkinje cells of the cerebellum. Ubiquitin mRNA is elevated after axotomy in the adult, suggesting an enhanced requirement for ubiquitin during axonal regeneration. Hence, increased protein ubiquitination appears to be particularly prominent during neuronal process formation and remodeling.

Recent studies addressed the function of ubiquitination during neuronal development. One of the most widely used in vitro models of neuronal differentiation is the pheochromocytoma PC12 cell line, which extends processes and acquires various neuron-like characteristics in response to treatment with nerve growth factor (NGF). NGF-mediated neurite outgrowth of PC12 cells is accompanied by 1) increased levels of high-molecular-weight ubiquitin conjugates in PC12 nuclei, 2) decreased levels of free ubiquitin in cytoplasmic and nuclear extracts, 3) upregulation of at least four E2 activities, and 4) enhanced capacities for ubiquitination (16). However, proteasomal degradation rates are not elevated compared with control cells (12). These findings suggest that, upon neuronal differentiation, proteins with nuclear functions during proliferation are ubiquitinated at an enhanced rate and targeted for degradation. Nevertheless, it is also possible that ubiquitinated proteins act as positive regulators of neurite outgrowth.

To further dissect the role of the UPS in neuronal differentiation, inhibitors of a specific pathway involving E2-14kD and E3 activities characterized as "N-end-rule" protein degradation were applied (12). E2-14kD is highly homologous to the bendless gene (ben), which was originally isolated as a fruit fly missense mutation affecting the escape jump response of Drosophila melanogaster. This pathway is implicated in the cleavage of proteins with destabilizing amino-terminal residues that include bulky hydrophobic (e.g., leucine, tryptophan, or phenylalanine) or basic amino residues (e.g., arginine, histidine, or lysine). N-end-rule-mediated ubiquitination is apparently increased during neuronal differentiation, and dipeptide inhibitors of this pathway (e.g., Leu-Ala) suppress NGF- and basic fibroblast growth factor-induced neurite outgrowth in PC12 cells as well as in amphibian neuroepithelial cells, whereas the addition of the reverse dipeptide (Ala-Leu) does not exert any inhibitory effect (8).

Various E3s have been detected in the nervous system. Among them is the anaphase-promoting complex (APC), a ubiquitin ligase that targets cyclin B for degradation at the end of mitosis. This ligase is kept active in association with CDH1, a substrate-specific subunit of APC. Both APC and CDH1 are detected in nuclei of postmitotic neurons in all brain regions, indicating important functions beyond the control of cell cycle progression. Other ubiquitin ligases have primarily been found in their mutated form, causing rare, inheritable neurological diseases. Prominent examples include MID1, a microtubule-binding protein that, if mutated, causes X-linked Opitz G/BBB syndrome (17). Opitz syndrome is characterized by ventral midline defects and is associated with mental retardation and various other abnormalities. Mutated MID1 causes accumulation of the catalytic subunit of protein phosphatase 2A, resulting in hypophosphorylation of microtubule-associated proteins. Another congenital disorder, Angelman syndrome, is caused by mutations in the E6-AP gene, which encodes a HECT ubiquitin ligase. Affected children exhibit movement disturbances, are unable to speak, and are mentally retarded. Finally, the ubiquitin ligase parkin (15) has been studied extensively in the brain because of its involvement in autosomal recessive Parkinson’s disease (see below).

	Regulation and function of the proteasome in neurons

Top Introduction Relevance of ubiquitination for... Regulation and function of... Involvement of the UPS... Conclusions References

 
Proteasomal subunits are encoded by a considerable number of genes that are differentially expressed between organs, i.e., proteasomes from the brain are different from those found in the spleen (11). Within the brain, immunoreactivity against the 20S proteasome particle is heterogeneously located in all areas, with predominant staining of large motor neuron cell bodies (10). Dendritic and axonal processes, including their synaptic terminals, contain proteasomes, indicating a functional role of localized protein degradation in various aspects of neuronal morphology.

Cytosolic proteasomal activity accounts for most of the total activity, although nuclei exhibit strong immunoreactivity for proteasomal subunits in the brain. Some subunit mRNAs are predominantly synthesized in specific areas. C2 mRNA is highly expressed in the cerebellum, whereas PA28ß is mainly found in the striatum and brain stem, in addition to its strong synthesis in the cerebellum (4). PA28 and the -subunit C6 mRNA are developmentally regulated in the rat midbrain, with highest expression in the early postnatal phase (5). C2 mRNA is significantly decreased contralateral to an ischemic lesion in the rat brain, and proteasomal subunits with catalytic activities are regulated upon neuronal differentiation in human neuronal progenitor cells as well as in microglial cells by interferon- and other inflammatory agents, resembling the exchange of subunits in antigen-presenting cells of the immune system. Therefore, proteasomal subunit composition can be modulated in response to the specific needs of neuronal and glial cells.

The role of the proteasome in the nervous system was initially studied in neuronal cell lines by using specific proteasome inhibitors. During the early period of treatment with membrane-permeable and irreversible inhibitors like lactacystin cells, cytoplasmic processes are extended, reflecting early stages of neuronal differentiation. Neuroblastoma cells acquire a bipolar morphology and become multipolar later on. Similar observations have been made in PC12 cells (12,13), suggesting that in tumor cells proteasomal inhibition shifts the balance of cell cycle-associated proteins required for proliferation and proteins necessary for differentiation to the latter. Inhibition of other proteases like calpain does not promote neurite outgrowth, indicating the specificity of the lactacystin response. Interestingly, the effects of proteasome inhibition on neurite outgrowth do not require ERK or phosphatidylinositol 3-kinase, although ERK activities are induced in PC12 cells treated with lactacystin or acetyl-Leu-Leu-norleucinal. Instead, proteasome inhibition may utilize stress-activated protein kinase to induce differentiation. Hence, regulating protein levels via modification of proteasomal activity may play an important role in neuronal development.

From studies using fluorogenic peptide substrates of the proteasome, it became clear that proteasomal activity is inhibited during aging and under various pathological conditions, such as Parkinson’s, Lewy bodies, or Alzheimer’s disease, as well as ischemia-reperfusion injury (4). Prolonged inhibition of proteasomal activity causes accumulation of a number of proteins, resulting in neuronal vulnerability. These include proteins associated with apoptosis, e.g., cyclin D1, a marker of the G₁/S transition of the cell cycle that induces cell death when overexpressed in neuronal lines. Furthermore, proteins relevant for the pathogenesis of neurological diseases are elevated in the presence of proteasome inhibitors, among them presenilin, hyperphosphorylated neurofilaments, ß-amyloid peptide, and parkin. A vicious cycle probably starts when accumulated proteins block the internal compartment of the proteasome, thereby further reducing proteasomal activity and initiating the cell death program. On the other hand, the pathogenetic mechanism of neuronal apoptosis in neurodegenerative diseases may be far more complex, because blockade of ubiquitination or of the proteasome may also prevent the neurotoxicity of accumulated proteins like the ß-amyloid peptide.

Various indicators of neuronal apoptosis, including chromatin fragmentation, DNA laddering, caspase activation, poly ADP-ribosylation, and release of cytochrome c, have been observed in response to proteasome inhibition. Indeed, it is not surprising that complete blockade of the proteasome by lactacystin ultimately arrests cellular metabolism and causes cell death. However, depending on the cell type and the concentration of the respective proteasomal inhibitor, the onset of apoptosis is delayed in some neuronal in vitro models, probably because proteasomal activity is required for the activation of proapoptotic signaling intermediates. It has been shown that lactacystin blocks caspase-1-mediated processing of poly(ADP-ribose) polymerase, thus prolonging survival of postmitotic NGF-deprived sympathetic neurons. Furthermore, proteasome inhibitors prevent early caspase-3 activation and tau cleavage in cerebellar granule neurons undergoing cell death induced by reduced extracellular potassium (2). In this model, however, UPS function is significantly impaired in the later, execution phase of neuronal apoptosis, with accumulation of ubiquitinated proteins and impairment of the proteasomal trypsin- and chymotrypsin-like activities.

"Reactive oxygen species have been suggested to play an important role in proteasome inhibition...."

Posttranslational modifications of the proteasome are likely to play a role in impaired proteasome function, because decreased activity is not accompanied by reduced total proteasome content (9). Reactive oxygen species have been suggested to play an important role in proteasome inhibition, probably by increasing the level of lipid peroxidation products followed by protein cross-linking. Aggregated proteins may then physically obstruct proteasome entry or block the catalytic sites within the ß-rings of the central core. Neurons are probably protected to some degree against high concentrations of reactive oxygen species by increasing levels of heat shock proteins, which aid in folding, trafficking, or disaggregation of proteins on their way to the proteasome. Other mechanisms may include reversible blockade of the proteasome for short periods of time by physiological modulators (like PI31) to promote degradation of oxidized proteins in the lysosomal pathway instead. It should be pointed out, however, that reactive oxygen species (e.g., nitric oxide) serve a number of essential intracellular functions as signaling intermediates and may even enhance proteasomal activity when concentrations are moderately increased.

	Involvement of the UPS in inclusion body formation and neurological disease

Top Introduction Relevance of ubiquitination for... Regulation and function of... Involvement of the UPS... Conclusions References

 
Neurodegenerative disorders like Alzheimer’s disease and dementia with Lewy bodies account for the cognitive decline of the majority of elderly patients. Both diseases are characterized by the accumulation of intraneuronal inclusions, which may be regarded as neuropathological hallmark of other human chronic neurodegenerative disorders as well. The proteinaceous, insoluble aggregates ("aggresomes") are found in the neuronal cytoplasm, axoplasm, or neuronal nuclei and may be encapsulated by intermediate filaments, resulting in filamentous deposits. Recent evidence suggests that they are formed through cross-linking of various hydrophobic regions exposed by several mechanisms (e.g., mutation, misfolding, or oxidative damage) and retrogradely transported along microtubules to the centrosome.

The importance of the UPS in inclusion body formation was elucidated when a variety of molecules involved in ubiquitin-mediated protein degradation, among them proteasomal subunits and other components of the UPS, were detected in inclusion bodies. Increasing evidence suggests that proteins forming aggregates either resist or inhibit proteolysis via the UPS. Mutations that impair normal ubiquitination (or deubiquitination) lead to protein accumulation, resulting in proteasomal inhibition (1), and, conversely, inhibition of the proteasome using membrane-permeable and irreversible blockers of the proteasomal catalytic activities results in aggresome formation (Fig. 2).

View larger version (48K): [in this window] [in a new window]   FIGURE 2. Dissociated sympathetic neurons treated with the proteasome inhibitor lactacystin (10 µM) for 24 h. Note the phase-dark cytoplasmic inclusion bodies (arrows) in the large, viable neurons with intact nuclei (as revealed by Hoechst staining). Degenerated neurons exhibiting apoptotic nuclei do not contain inclusions. Bar = 15 µm.

Ubiquitin immunoreactivity is detected within intranuclear inclusions and dystrophic neuronal processes in patients with Huntington’s disease, suggesting that abnormal huntingtin is targeted for proteolysis but resistant to effective removal (18).

Other neurodegenerative disorders reveal similar neuropathological findings, for example, Lewy bodies not only contain lipids and neurofilaments but also ubiquitin and other proteins, which have been identified as the cause of familial forms of Parkinson’s disease including mutations of -synuclein. The rare Ile93Met mutation in ubiquitin carboxy-terminal hydrolase (UchL1) results in Parkinson’s disease probably by decreasing the activity of L1. It is likely that failure of protein ubiquitination or deubiquitination as well as general proteasome inhibition make catecholaminergic neurons particularly vulnerable to cellular stress and to disturbances in the balance of the redox system.

Mutations in parkin cause an autosomal recessive form of Parkinson’s disease (arPD) (15). Parkin is a member of the E3 family with two RING fingers at its carboxy-terminal end separated by an in-between RING. Interestingly, parkin is also a ubiquitin-domain protein because a ubiquitin-like sequence is located in the amino terminal of parkin. Substrates of parkin are being identified, e.g., the turnover of cell division control protein (CDCrel-1), a member of the septin GTPases probably involved in dopamine release, is impaired in arPD patients (20). Another substrate, the parkin-associated endothelin-receptor-like receptor (Pael-R), has been found to elicit cell death because it becomes unfolded and insoluble when overexpressed, whereas coexpression of intact parkin protects against death. Other substrates of parkin have been identified, including the glycosylated form of -synuclein (-Sp22) and synphilin-1 (6). -Sp22 is associated with parkin as well as with UbcH7 and accumulates in arPD patients in its nonubiquitinated form, suggesting a common pathogenetic mechanism of parkin- and -synuclein-associated Parkinson’s disease. It appears that all three components, parkin, synphilin-1, and -synuclein, are required for the formation of Lewy bodies.

Mutations in E3s like parkin reduce the formation of ubiquitin-positive inclusions, suggesting that segregation of abnormal proteins requires ubiquitination and serves as a natural defense mechanism to protect cellular structures from the cytotoxic effects of abnormal proteins that cannot be recognized and degraded by the proteasome. This hypothesis is further supported by the frequent occurrence of Lewy bodies in late-onset sporadic Parkinson’s disease, whereas they are rarely found in patients with the young-onset familial form of Parkinson’s disease that is often accompanied by severe neurodegeneration. The high number of cases with incidental Lewy bodies and mild neurodegeneration in the elderly probably represents a preclinical stage of Parkinson’s disease that is characterized by a still-intact ability of the neuron to compartmentalize abnormal or cytotoxic proteins as insoluble aggregates in inclusions (Fig. 1). The assumption that the UPS promotes inclusion body formation to reduce the toxicity of misfolded, mutant, or otherwise damaged proteins in their soluble form is corroborated by various studies that clearly demonstrate enhanced toxicity of mutant proteins [ataxin-1 (which cannot self-aggregate) or mutant huntingtin] in neuronal cultures under conditions that prevent ubiquitination and inclusion formation (14).

	Conclusions

Top Introduction Relevance of ubiquitination for... Regulation and function of... Involvement of the UPS... Conclusions References

 
Beyond its role as the major intracellular protein degradation machinery, the UPS has been identified as an important regulator of protein activation, endocytosis, and transcriptional regulation. In the nervous system, the physiological significance of this pathway is just beginning to be explored. Dissecting the role of the UPS during neuronal differentiation and neuronal apoptosis in cell culture models has laid the groundwork for our current understanding of ubiquitination and proteasomal protein degradation in the brain. The UPS is clearly involved in the pathogenesis of various neurodegenerative disorders like Parkinson’s disease. Familial forms of Parkinson’s disease are associated with mutations of UPS components or of proteins like -synuclein that may resist proteasomal degradation if mutated. By segregating these proteins into aggresomes, neurons may have developed a mechanism to prevent the toxicity of their soluble or protofibrillary forms. The high sensitivity of postmitotic, fully differentiated cells to oxidized or misfolded proteins, which need to be eliminated rapidly to maintain neuronal metabolism, makes neurons particularly vulnerable to defects in ubiquitin-mediated protein degradation. Therefore, future therapeutic strategies will have to aim at the repair and possibly augmentation of the neuronal UPS, which will impose a major challenge.

	Acknowledgments

 
I wish to thank Rainer Schneider for critical suggestions on the manuscript, and I apologize to all contributors to the field whose work has not been cited due to space limitations.

	References

Top Introduction Relevance of ubiquitination for... Regulation and function of... Involvement of the UPS... Conclusions References

 

1. Bence NF, Sampat RM, and Kopito RR. Impairment of the ubiquitin-proteasome system by protein aggregation. Science 292: 1552–1555, 2001.[Abstract/Free Full Text]
2. Canu N, Barbato C, Ciotti MT, Serafino A, Dus L, and Calissano P. Proteasome involvement and accumulation of ubiquitinated proteins in cerebellar granule neurons undergoing apoptosis. J Neurosci 20: 589–599, 2000.[Abstract/Free Full Text]
3. Cummings CJ, Reinstein E, Sun Y, Antalffy B, Jiang Y, Ciechanover A, Orr HT, Beaudet AL, and Zoghbi HY. Mutation of the E6-AP ubiquitin ligase reduces nuclear inclusion frequency while accelerating polyglutamine-induced pathology in SCA1 mice. Neuron 24: 879–892, 1999.[Web of Science][Medline]
4. Ding Q and Keller JN. Proteasomes and proteasome inhibition in the central nervous system. Free Radic Biol Med 31: 574–584, 2001.[Web of Science][Medline]
5. El-Khodor BF, Kholodilov NG, Yarygina O, and Burke RE. The expression of mRNAs for the proteasome complex is developmentally regulated in the rat mesencephalon. Brain Res Dev Brain Res 129: 47–56, 2001.[Medline]
6. Engelender S, Kaminsky Z, Guo X, Sharp AH, Amaravi RK, Kleiderlein JJ, Margolis RL, Troncoso JC, Lanahan AA, Worley PF, Dawson VL, Dawson TM, and Ross CA. Synphilin-1 associates with alpha-synuclein and promotes the formation of cytosolic inclusions. Nat Genet 22: 110–114, 1999.[Web of Science][Medline]
7. Hegde AN, Inokuchi K, Pei W, Casadio A, Ghirardi M, Chain DG, Martin KC, Kandel ER, and Schwartz JH. Ubiquitin C-terminal hydrolase is an immediate-early gene essential for long-term facilitation in Aplysia. Cell 89: 115–126, 1997.[Web of Science][Medline]
8. Hondermarck H, Sy J, Bradshaw RA, and Arfin SM. Dipeptide inhibitors of ubiquitin-mediated protein turnover prevent growth factor-induced neurite outgrowth in rat pheochromocytoma PC12 cells. Biochem Biophys Res Commun 189: 280–288, 1992.[Web of Science][Medline]
9. Keller JN, Huang FF, Zhu H, Yu J, Ho YS, and Kindy TS. Oxidative stress-associated impairment of proteasome activity during ischemia-reperfusion injury. J Cereb Blood Flow Metab 20: 1467–1473, 2000.[Web of Science][Medline]
10. Mengual E, Arizti P, Rodrigo J, Gimenez-Amaya JM, and Castano JG. Immunohistochemical distribution and electron microscopic subcellular localization of the proteasome in the rat CNS. J Neurosci 16: 6331–6341, 1996.[Abstract/Free Full Text]
11. Noda C, Tanahashi N, Shimbara N, Hendil KB, and Tanaka K. Tissue distribution of constitutive proteasomes, immunoproteasomes, and PA28 in rats. Biochem Biophys Res Commun 277: 348–354, 2000.[Medline]
12. Obin M, Mesco E, Gong X, Haas AL, Joseph J, and Taylor A. Neurite outgrowth in PC12 cells. Distinguishing the roles of ubiquitylation and ubiquitin-dependent proteolysis. J Biol Chem 274: 11789–11795, 1999.[Abstract/Free Full Text]
13. Ohtani-Kaneko R, Takada K, Iigo M, Hara M, Yokosawa H, Kawashima S, Ohkawa K, and Hirata K. Proteasome inhibitors which induce neurite outgrowth from PC12h cells cause different subcellular accumulations of multi-ubiquitin chains. Neurochem Res 23: 1435–1443, 1998.[Medline]
14. Saudou F, Finkbeiner S, Devys D, and Greenberg ME. Huntingtin acts in the nucleus to induce apoptosis but death does not correlate with the formation of intranuclear inclusions. Cell 95: 55–66, 1998.[Web of Science][Medline]
15. Shimura H, Hattori N, Kubo S, Mizuno Y, Asakawa S, Minoshima S, Shimizu N, Iwai K, Chiba T, Tanaka K, and Suzuki T. Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase. Nat Genet 25: 302–305, 2000.[Web of Science][Medline]
16. Takada K, Kanda T, Ohkawa K, and Matsuda M. Ubiquitin and ubiquitin-protein conjugates in PC12h cells: changes during neuronal differentiation. Neurochem Res 19: 391–398, 1994.[Medline]
17. Trockenbacher A, Suckow V, Foerster J, Winter J, Krauss S, Ropers HH, Schneider R, and Schweiger S. MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation. Nat Genet 29: 287–294, 2001.[Web of Science][Medline]
18. Waelter S, Boeddrich A, Lurz R, Scherzinger E, Lueder G, Lehrach H, and Wanker EE. Accumulation of mutant huntingtin fragments in aggresome-like inclusion bodies as a result of insufficient protein degradation. Mol Biol Cell 12: 1393–1407, 2001.[Abstract/Free Full Text]
19. Weissman AM. Themes and variations on ubiquitylation. Nat Rev Mol Cell Biol 2: 169–178, 2001.[Web of Science][Medline]
20. Zhang Y, Gao J, Chung KK, Huang H, Dawson VL, and Dawson TM. Parkin functions as an E2-dependent ubiquitin-protein ligase and promotes the degradation of the synaptic vesicle-associated protein, CDCrel-1. Proc Natl Acad Sci USA 97: 13354–13359, 2000.[Abstract/Free Full Text]

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